Composite

Part:BBa_K3726055

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)


L1_MCGII-A

This is a composite part that corresponds with a level 1 MoClo part in accordance with the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726038” L0_MCGII which contains the other half part of the MCG cycle.

The other half of the MCG cycle is going to be expressed in the part PKMCGII_Lv1 which has two variants “BBa_K3726053” L1_PKMCGII-A and “BBa_K3726054” L1_PKMCGII-B. The Malate-acetyl-CoA-Glycerate (MCG) cycle aims to the production of acetyl-CoA with high efficiency coupled to carbon capture from bicarbonate anion and with recovery of metabolic intermediates produced during photorespiration. (H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021).

The part is flanked by two homology regions “ BBa_K3726110” 5CON1(H)_nNS4-up (PCC 11801) and “BBa_K3726111” 3CON5(H)_nNS4-down (PCC 11801) for the double homologous recombination within the genome of PCC 11801.

Likewise, an ampicillin resistance cassette is included downstream the transcriptional unit region.

Expression is controlled by the strong constitutive promoter Prbcl-m36 “BBa_K3726080” and the strong synthetic RBS, RBS_Ppc “BBa_K3726091”.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal PstI site found at 1089
    Illegal PstI site found at 2877
    Illegal PstI site found at 2976
    Illegal PstI site found at 3561
    Illegal PstI site found at 4519
    Illegal PstI site found at 4747
    Illegal PstI site found at 6257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal NheI site found at 5831
    Illegal PstI site found at 1089
    Illegal PstI site found at 2877
    Illegal PstI site found at 2976
    Illegal PstI site found at 3561
    Illegal PstI site found at 4519
    Illegal PstI site found at 4747
    Illegal PstI site found at 6257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal BglII site found at 1234
    Illegal BglII site found at 3274
    Illegal BglII site found at 4638
    Illegal BamHI site found at 3372
    Illegal BamHI site found at 3387
    Illegal BamHI site found at 7086
    Illegal BamHI site found at 7544
    Illegal BamHI site found at 8517
    Illegal XhoI site found at 337
    Illegal XhoI site found at 2064
    Illegal XhoI site found at 2709
    Illegal XhoI site found at 3183
    Illegal XhoI site found at 5713
    Illegal XhoI site found at 6380
    Illegal XhoI site found at 6513
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal PstI site found at 1089
    Illegal PstI site found at 2877
    Illegal PstI site found at 2976
    Illegal PstI site found at 3561
    Illegal PstI site found at 4519
    Illegal PstI site found at 4747
    Illegal PstI site found at 6257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal PstI site found at 1089
    Illegal PstI site found at 2877
    Illegal PstI site found at 2976
    Illegal PstI site found at 3561
    Illegal PstI site found at 4519
    Illegal PstI site found at 4747
    Illegal PstI site found at 6257
    Illegal NgoMIV site found at 2820
    Illegal NgoMIV site found at 4209
    Illegal NgoMIV site found at 4383
    Illegal NgoMIV site found at 4533
    Illegal NgoMIV site found at 7707
    Illegal AgeI site found at 4834
  • 1000
    COMPATIBLE WITH RFC[1000]


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